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Adhesion and growth of adipose tissue-derived stem cells on fibrin assemblies with attached growth factors for tissue engineering of heart valves
Filová, Elena ; Trávníčková, Martina ; Knitlová, Jarmila ; Matějka, Roman ; Kučerová, Johanka ; Riedelová, Zuzana ; Brynda, Eduard ; Bačáková, Lucie
Currently used xenogeneic biological heart valve prostheses are decellularized and crosslinked with glutaraldehyde. These grafts usually undergo degeneration and calcification. Pericardium-based heart valve prostheses, re-seeded with autologous cells, i.e. Adipose tissue-derived cells (ASCs) and endothelial cells, could have longer durability and biocompatibility. In order to improve the adhesion of cells and their ingrowth into decellularized pericardium, various fibrin (Fb) layers were developed, i.e. Fb, Fb with covalently bound heparin (H), Fb with either vascular endothelial growth factor (VEGF) or fibroblast growth factor 2 (FGF) in various concentrations (1 ng/ml, 10 ng/ml, 100 ng/ml) or with both VEGF and FGF (100 ng/ml). Growth factors were attached onto Fb via heparin or were adsorbed. ASCs were seeded on theses layers in a DMEM medium supplemented with 2 % of fetal bovine serum, TGFβ1 and BMP-4 (both 2.5 ng/ml), and with ascorbic acid. Cell adhesion and growth/viability was assessed by counted cell number/MTS evaluation. ASCs were stained for differentiation markers of smooth muscle cells, such as alpha-actin, calponin, and myosin heavy chain. On day 7, ASCs on Fb-H-VEGF layers produced both calponin and alpha-actin. An increased FGF concentration caused reduced calponin staining of ASCs. Lack of heparin in fibrin assemblies with growth factors inhibited the production of both alpha-actin and calponin in ASCs. The highest ASCs density/viability was found on Fb-H-VEGF-FGF layer. The proper formulation of fibrin coatings could be favorable for ASCs growth and differentiation and could subsequently support endothelialization of cardiovascular prostheses with endothelial cells.
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Periopathogens and systemic markers in etiology and diagnostics of periodontal and cardiovascular diseases.
Myšák, Jaroslav ; Foltán, René (advisor) ; Izakovičová Hollá, Lydie (referee) ; Markovská, Neda (referee)
Periodontitis is a disease primarily affecting tooth attachment, i.e. concerning oral cavity, however, its connection to a number of systemic diseases is apparent nowadays. Oral microorganisms and their interaction with the immune system of their host play a significant part in the etiology of this multifactorial disease. Rapid development of DNA-based diagnostic methods in the last 15 years dramatically increased the spectrum of identified oral microorganisms and promoted understanding of how particular taxons correlate with periodontal health or disease. Moreover, next generation sequencing methods also bring new possibilities to study the relationship between periodontitis and other diseases such as diabetes mellitus, cardiovascular diseases (CVD), stroke, lung infection or kidney diseases, etc. The presented work focuses on the use of sequencing methods to compare the taxonomic composition of microbiomes within oral cavity and the tissues of aortic valves in patients suffering from CVD. The characterisation of taxonomic composition of microbiome in the analysed tissues was performed using the method of 454 pyrosequencing of variable region IV-V of the bacterial 16S rDNA. The present taxons were determined by comparing the obtained sequences with the Human Oral Microbiome Database. The common...
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The issue of compensation of a heart valve from the perspective of the patient
MUNDLOVÁ, Anna
A heart valve replacement is one of the most technically difficult cardiac surgeries. For the patient, it means a mental and physical health intervention. Heart valve surgery is currently the second most common cardiac surgery - accounting for up to 20% of the total number of cardiac surgeries. The goals and objectives of a heart valve replacement are to improve patients' quality of life and their medical prognosis. The heart valve replacement is carried out only in cases when we cannot perform the heart valve plastic surgery and thereby replace the entire heart valve with an artificial one. Heart valve prosthetics can be mechanical - patients with this kind of prosthetics are then warfarinized for life or they can also be made of biological material (bioprosthetics). Patients with bioprostehtics require only a three month long anticoagulant therapy. Temporary postoperative complications may occur such as dyspnoea, gastric and intestinal atony or aggravation of pulmonary functions. Among the rarer complications are rhythm disorders, thrombosis of prosthetic heart valve, and last but not least infective endocarditis. The patients, after the heart valve replacement, are at a greater risk of complications, therefore they are obliged to always have their patient at risk of infection endocarditis card with them (see appendix 4). After the operation the patients are informed about the lifestyle changes they are to follow within their daily routine, the information is delivered by doctors and nurses. The task of the nurses is to inform the patient about the lifestyle changes together with doctors. Good understanding and observance of these lifestyle changes help to improve patients' life after the heart valve replacement. This bachelor thesis is divided into an empirical and theoretical part. The theoretical part focussing on the problems connected with the heart valve replacement, valvular imperfections, perioperative nursing, patients' lifestyle changes and their quality of life.
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